CALB aan dhaqdhaqaaq lahayn
CALB waxaa lagu joojiyaa nuugista jireed ee resin-ka aadka u hydrophobic-ka ah kaas oo ah polymer styrene/methacrylate ah oo macroporous ah. CALB-ka aan dhaqdhaqaaqin wuxuu ku habboon yahay isticmaalka dareeraha dabiiciga ah iyo nidaamyada aan dareeraha lahayn, waxaana dib loo warshadeyn karaa oo dib loo isticmaali karaa waqtiyo badan xaaladaha ku habboon.
Xeerka Alaabta: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.
★ Firfircooni sare, xulasho sare oo chiral ah iyo xasillooni sare.
★Waxqabad wanaagsan marxaladaha aan biyaha lahayn.
★Si fudud uga saar nidaamka falcelinta, si degdeg ah u jooji falcelinta, oo iska ilaali haraaga borotiinka ee ku jira badeecada.
★Waa la dib u warshadayn karaa oo dib ayaa loo isticmaali karaa si loo yareeyo kharashka.
| Hawl | ≥10000PLU/g |
| Kala duwanaanshaha pH ee falcelinta | 5-9 |
| Heerkulka falcelinta | 10-60℃ |
| Muuqaalka | CALB-IMMO100-A: Jaalle khafiif ah ilaa bunni adag CALB-IMMO100-B: Cad ilaa bunni khafiif ah oo adag |
| Cabbirka walxaha | 300-500μm |
| Lumis marka la qalajiyo 105℃ | 0.5%-3.0% |
| Xabagta loogu talagalay joojinta dhaqdhaqaaqa | Polymer-ka macroporous, styrene/methacrylate |
| Dareeraha falcelinta | Biyo, dareere dabiici ah, iwm., ama aan lahayn dareere. Falcelinta ku jirta qaar ka mid ah dareere dabiici ah, 3% biyo ayaa lagu dari karaa si loo hagaajiyo saameynta falgalka. |
| Cabbirka walxaha | CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm |
Qeexitaanka cutubka: 1 cutub wuxuu u dhigmaa isku-darka 1μmol daqiiqaddii propyl laurate laga soo bilaabo lauric acid iyo 1-propanol 60℃. CALB-IMMP100-A iyo CALB-IMMO100-B ee kor ku xusan waxay u dhigmaan sideyaasha aan dhaqdhaqaaqa lahayn ee leh cabbirro kala duwan oo walxaha ah.
1. Nooca fal-galiyaha
Ensaymka aan dhaqdhaqaaq lahayn wuxuu khuseeyaa labadaba fal-galiyaha qaybta kettle-ka iyo fal-galiyaha socodka joogtada ah ee sariirta go'an. Waa in la ogaadaa in laga fogaado burburinta xoogga dibadda inta lagu jiro quudinta ama buuxinta.
2. Falcelinta pH, heerkulka iyo dareeraha
Ensaymka aan dhaqdhaqaaqin waa in lagu daraa ugu dambeeya, ka dib marka walxaha kale lagu daro oo la milmo, oo pH-ka la hagaajiyo.
Haddii isticmaalka substrate-ka ama sameynta badeecaddu ay horseedi doonto isbeddelka pH inta lagu jiro falcelinta, waa in lagu daraa kayd ku filan nidaamka falcelinta, ama pH-ga waa in la kormeeraa oo la hagaajiyaa inta lagu jiro falcelinta.
Inta u dhaxaysa heerka dulqaadka heerkulka ee CALB (ka hooseeya 60 ℃), heerka beddelka ayaa kordhay iyadoo heerkulku kor u kacayo. Isticmaalka wax ku oolka ah, heerkulka falcelinta waa in loo doortaa iyadoo loo eegayo xasilloonida substrate-ka ama badeecada.
Guud ahaan, falgalka hydrolysis-ka ester-ku wuxuu ku habboon yahay nidaamka marxaladda biyaha, halka falgalka synthesis-ka ester-ku uu ku habboon yahay nidaamka marxaladda dabiiciga ah. Dareeraha dabiiciga ah wuxuu noqon karaa ethanol, tetrahydrofuran, n-hexane, n-heptane iyo toluene, ama dareere isku dhafan oo ku habboon. Falcelinta ku jirta qaar ka mid ah dareereyaasha dabiiciga ah, 3% biyo ayaa lagu dari karaa si loo hagaajiyo saameynta falgalka.
3. Dib-u-isticmaalka iyo cimriga adeegga CALB
Xaaladda falcelinta ku habboon, CALB waa la soo celin karaa oo dib ayaa loo isticmaali karaa, waqtiyada gaarka ahna way ku kala duwan yihiin mashaariicda kala duwan.
Haddii CALB-ka la soo celiyey aan si joogto ah dib loogu isticmaalin oo loo baahan yahay in la kaydiyo ka dib soo kabashada, waa in la dhaqo oo la qalajiyaa oo lagu xiraa heerkul ah 2-8 ℃.
Ka dib dhowr wareeg oo dib loo isticmaali karo, haddii waxtarka falgalka uu yara yaraado, CALB si habboon ayaa loogu dari karaa oo loo sii wadi karaa isticmaalka. Haddii waxtarka falgalka si dhab ah loo yareeyo, waa in la beddelaa.
Tusaale 1 (Aminolysis)(1):
Tusaale 2 (Aminolysis)(2):
Tusaale 3 (Isku-darka polyester-ka furitaanka giraanta)(3):
Tusaale 4(Transesterification, regioselective ee kooxda hydroxyl)(4):
Tusaale 5 (Transesterification, xallinta kinetic-ga ee khamriga jinsiyadda)(5):
Tusaale 6(Esterification, xallinta kinetic ee carboxylic acid)(6):
Tusaale 7 (Esterolysis, xallinta kinetic)(7):
Tusaale 8(Hydrolysis-ka amides)(8):
Tusaale 9 (Dhaqdhaqaaqa amines-ka)(9):
Tusaale 10 (falcelinta ku darista Aza-Michael)(10):
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