Lactate Dehydrogenase (LDH)
Sharaxaad Gaaban:
Ku saabsan Lactate dehydrogenase
Waxaa jira 1 nooc oo ah wax soo saarka ensaymka LDH (Lambarka sida ES-LDH) oo ay samaysay SyncoZymes. LDH waxay kicin kartaa dhimista pyruvate una beddelaysaa L-lactic acid, ama dhimista asiidhyada kale ee α-keto ee la midka ah. Inta lagu jiro habka katalisnimada, NADH waa lagama maarmaan sida qaadaha haydarojiin.
Nooca falgalka katalytic-ga ee lactate dehydrogenase
Moobayl/Wechat/WhatsApp: +86-13681683526
Iimayl:lchen@syncozymes.com
| Ensaymyada | Koodhka Badeecada | Faahfaahinta |
| Budada Ensaymka | ES-LDH-101 | set 1 Monoamine Oxidase ah, 50 mg midkiiba 1 shay * 50mg / shay, ama tiro kale |
Nidaamka falcelinta soo socda ayaa loo isticmaali karaa tixraaca:
1-10 mg/ml: Substrate
10 mg/ml: Ensaym
10% (v/v): Isku-darka dabiiciga ah (1)
100 mM: Baafiyaha Fosfate (pH7.0)
0.2 mg/ml: NAD+
100 mM: Qaab-dhismeedka Ammonium
10 mg/ml: Qaabaynta dehydrogenase (FDH)
Ku shid falgallada mashiinka heerkulka joogtada ah (tusaale ahaan, 30oC, 150rpm) muddo 24 ~ 48 saacadood ah. Ku shub isku dar kasta si uu borotiinka u soo daadiyo, kuwaas oo ah kuwa ugu sarreeya waxaa loo isticmaali karaa falanqaynta TLC, HPLC ama GC.
Fiiro gaar ah (1): Dareeraha dabiiciga ah (sida DMSO, methanol, acetonitrile, IPA, iwm.) waa ikhtiyaari, waxaana loo isticmaalaa substrate-yada aan milmi karin.
