Nitrile hydratase (NHT)
Sharaxaad Gaaban:
Ku saabsan ES-NHTs
ES-NHTs: waa nooc ka mid ah metalloenzyme, kaas oo si xushmad leh u fuuqbixiya nitriles-ka u dhigma amide aasaasiga ah.Waxaa si ballaaran loo isticmaalaa ma aha oo kaliya loogu talagalay isku dhafka amino acids, amides, acids karboksylic iyo kuwa ka soo jeeda, laakiin sidoo kale diyaarinta daawooyinka chiral.Waxaa jira 24 nooc oo nitrile hydratases ah oo ay soo saartay SyncoZymes (Lambarka sida ES-NHT-101～ES-NHT-124).
Nooca falcelinta catalytic:
Mobile/Wechat/WhatsApp: +86-13681683526
iimayl:lchen@syncozymes.com
| Enzymes | Koodhka badeecada | Tilmaamid |
| Budada Enzyme | ES-NHT-101~ ES-NHT-124 | qayb ka mid ah 24 Nitrile Hydratases, 50 mg midkiiba 24 shay * 50mg / shay, ama tiro kale |
| Qalabka Baaritaanka (SynKit) | ES-NHT-2400 | qayb ka mid ah 24 Nnitrile Hydratases, 1 mg 24 shay kasta * 1mg / shay |
★ gaar ahaan substrate-ka sare.
★ Xulasho xoog leh oo chiral ah.
★ beddelaad sare.
★ Alaabooyin ka yar
★ Xaalado falcelin fudud.
★ deegaan ahaan.
➢ Caadiyan, habka falcelinta waa in lagu daraa substrate, xallinta wax-ka-qabashada (pH) iyo ES-NHT.
➢ Dhammaan ES-NHT-yada waxa lagu tijaabin karaa siday u kala horreeyaan habka falcelinta ee kore ama Qalabka Baadhista NHT (SynKit NHT).
➢ Dhammaan noocyada ES-NHT ee u dhigma xaaladaha kala duwan ee falcelinta ugu wanaagsan waa in si gaar ah loo darso.
➢ Substrate-ka sare u kaca ama alaabada leh ayaa laga yaabaa inay joojiyaan dhaqdhaqaaqa ES-NHT.Si kastaba ha ahaatee, xannibaadda waxaa lagu nafici karaa marka lagu daro qayb ka mid ah substrate, isku dhafka amidases ama soo saarista alaabta.
Tusaalaha 1 (isku-xidhka amidase)(1):
Tusaale 2(2):
Ka dhig 2 sano ka hooseeya -20 ℃.
Weligaa ha la xiriirin xaaladaha ba'an sida: heerkulka sare, pH sare/hoose iyo dareeraha organic-fiirsashada sare leh.
1 Vojtech V, Ludmila M, Alicja BV, iyo Tal.J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W.B, Thomas G, Christopher B.M, iyo tale.Tetrahedron lett, 2010, 51: 1639-1641.
