Phenylalanine dehydrogenase (PDH)
Sharaxaad Gaaban:
Ku saabsan Phenylalanine dehydrogenase
ES-PDH: Waa nooc ka mid ah oxidoreductase.Dareen-celinta togan waxay kicin kartaa deamination oksaydhiyaha ee cusbada L phenylalanine joogitaanka NAD si ay u soo saarto cusbada phenylpyruvate, falcelinta gadaashana waxay kicin kartaa isku dhafka milixda amino acid ee u dhigma ee cusbada phenylpyruvate.Syncozymes waxay soo saartay 8 shay oo ah phenylalanine dehydrogenase (lambareeyay ES-PDH-101~ES-PDH-108).ES-PDH waxaa loo isticmaali karaa in lagu diyaariyo cusbada phenylalanine ama milixda amino acid ee u dhigma ee laga helo cusbada fenylpyruvic
Nooca falcelinta catalytic:
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iimayl:lchen@syncozymes.com
| Enzymes | Koodhka badeecada | Tilmaamid |
| Budada Enzyme | ES-PDH-101~ ES-PDH-108 | 8 Nitrile Reductase, 50 mg 8 walxood * 50mg / shayga, ama tiro kale |
★ gaar ahaan substrate-ka sare.
★ Xulasho xoog leh oo chiral ah.
★ beddelaad sare.
★ Alaabooyin ka yar
★ Xaalado falcelin fudud.
★ deegaan ahaan.
➢ Caadiyan, habka falcelinta waa in ay ku jiraan substrate, xal buffer, enzyme, coenzyme (NAD(H)), nidaamka dib u soo kabashada coenzyme (tusaale ammonium formate iyo formate dehydrogenase).
➢ Nooc kasta oo ES-PDH ah oo u dhigma xaaladaha falcelinta ugu wanaagsan ee kala duwan, kaas oo si gaar ah loo baran karo haddii loo baahdo.
➢ ES-PDH waa in lagu daraa habka falcelinta si loo joogteeyo hawsha.
Tusaale 1 (Biosynthesis of phenylalanine derivatives ka phenylpyruvate derivatives)(1):
Ka dhig 2 sano ka hooseeya -20 ℃.
Weligaa ha la xiriirin xaaladaha ba'an sida: heerkulka sare, pH sare/hoose iyo dareeraha organic-fiirsashada sare leh.
1. Francesca Paradisi, Stuart Collins, iyo al.Wargeyska Biotechnology, 2007, 128, 408-411.
