Monoamine oxidase (MAO)
Enzymes | Koodhka badeecada | Tilmaamid |
Budada Enzyme | ES-MAO-101~ ES-MAO-105 | 5 Monoamine Oxidase, 50 mg 5 walxood * 50mg / shay, ama tiro kale |
Qalabka Baaritaanka (SynKit) | ES-MAO-500 | set of 5 Monoamine Oxidase, 1 mg midkiiba 5 shay * 1mg / shayga |
★ gaar ahaan substrate-ka sare.
★ Xulasho xoog leh oo chiral ah.
★ beddelaad sare.
★ Alaabooyin ka yar
★ Xaalado falcelin fudud
★ deegaan ahaan.
➢ Caadiyan, habka falcelinta waa in uu ku jiraa substrate, xal buffer, enzyme, coenzyme, iyo nidaamka dib u soo kicinta coenzyme.
➢ Dhammaan noocyada kala duwan ee MAO ee u dhigma xaaladaha kala duwan ee falcelinta ugu wanaagsan waa in si gaar ah loo darso.
➢ Substrate-ka sare u kaca ama alaabada leh ayaa laga yaabaa inay joojiyaan dhaqdhaqaaqa ES-MAO.Si kastaba ha ahaatee, xannibaadda waxaa lagu nafisi karaa marka lagu daro qayb ka mid ah substrate.
Tusaale 1 (Biosynthesis of (1R,5S) -6,6-dimethyl-3-aza-bicyclo[3.1.0] hex-2-ene)(1):
Ka dhig 2 sano ka hooseeya -20 ℃.
Weligaa ha la xiriirin xaaladaha ba'an sida: heerkulka sare, pH sare/hoose iyo dareeraha organic-fiirsashada sare leh.
1 Tao Li, Jack Liang, Alexandre Ambrogelly iyo al.J. AmChem.Soc.2012, 134, 6467-6472.