Transaminase (ATA)
Enzymes: Waa kicinta bayooloji ee makromolecular, inta badan enzymes waa borotiinno.
Transaminases: Waa nooc ka mid ah enzymes kuwaas oo kicinaya isku-gudbinta amino ee u dhexeeya amino acids iyo keto acids.Transaminases waa enzymes bayooloji oo muhiim ah oo ku jira isku-dhafka asymmetric iyo xallinta jinsiyadeed ee amines chiral.
Aminotransferase waxaa loo qaybin karaa afar fasal iyadoo loo eegayo isku xigxiga iyo qaabka: Ⅰ, Ⅱ, Ⅲ iyo Ⅳ.ω-aminotransferases waxay ka tirsan yihiin fasalka II transaminases, oo badanaa loo isticmaalo diyaarinta amines chiral iyo amino acids aan dabiici ahayn, sida β-amino acids.
ω-aminotransferases: Xaaladaha intooda badan, ω-transaminase waxaa loola jeedaa fasalka enzymes, taas oo falcelinta wareejinta ammonia ee catalytic iyada oo aan α-amino acid sida substrate ama alaabta.
Farsamaynta catalytic:
| Enzymes | Koodhka badeecada | Koodhka badeecada |
| Budada Enzyme | ES-ATA-101~ ES-ATA-165 | 65 ω-Transaminases, 50 mg midkiiba 65 shay * 50mg / shayga, ama tiro kale |
| Qalabka Baaritaanka (SynKit) | ES-ATA-6500 | 65 ω-Transaminases, 1 mg midkiiba 65 shay * 1mg / shay |
★ gaar ahaan substrate-ka sare.
★ Xulasho xoog leh oo chiral ah.
★ Waxtarka beddelka sare.
★ Alaabooyin ka yar
★ Xaalado falcelin fudud.
★ deegaan ahaan.
➢ Baadhitaanka enzyme waa in lagu sameeyaa substrates gaar ah sababtoo ah gaar ahaan substrate-ka, oo la helo ensaym ka kaca substrate-ka bartilmaameedka leh ee saamaynta ugu fiican.
➢ Weligaa ha la xiriirin xaaladaha ba'an sida: heerkulka sare, pH sare/hoose iyo dareere organic leh oo leh feejignaan sare.
Caadiyan, habka falcelinta waa in uu ku jiraa substrate, xal-u-helid, deeq-bixiye amino (sida amino acids iyo 1-phenyl ethylamine) ama reseptor (sida keto acids), coenzyme (PLP), cosolvent (sida DMSO).
ATA waa in lagu daraa ugu danbeyn habka falcelinta, ka dib marka pH iyo heerkulka lagu hagaajiyo xaalada falcelinta.
➢ Dhammaan noocyada ATA waxay leeyihiin xaalado falcelin oo wanaagsan oo kala duwan, sidaas darteed mid kasta oo iyaga ka mid ah waa in si gaar ah loo darso.
Tusaalaha 1-aad (isku-dhafka Sitagliptin, synthesis asymmetric)(1):
Tusaalaha 2 (Mexiletine, Isku-darka xallinta dhaqdhaqaaqa iyo isku-dhafka asymmetric)(2):
1 Savile CK, Janey JM, Mundorff EC, iyo al.Sayniska, 2010, 329 (16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, iyo al.Xarfaha dabiiciga ah, 2009,11 (21): 4810-4812.
