D-Amino acid dehydrogenase (D-AADH)
Sharaxaad Gaaban:
Ku saabsan D-Amino acid dehydrogenase
ES-D-AADH (D-Amino acid dehydrogenase): ensaymes oo yarayn kara 2-keto acid una dhigma D-amino acid.Dareen-celinta waxay u baahan tahay NADP (H) iyo ku-deeqaha ammonia-ga aan noolayn (sida ammonia ama ammonium ion).Waxaa jira 17 nooc oo ah D-amino acid dehydrogenases (Lambarka ES-DAADH-101~ ES-DAADH-117) oo ay soo saartay SyncoZymes.
Nooca falcelinta catalytic:
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| Enzymes | Koodhka badeecada | Tilmaamid |
| Budada Enzyme | ES-D-AADH-101~ ES-D-AADH-117 | 17 D-amino Acid Dehydrogenases, 50 mg 17 shay * 50mg / shay, ama tiro kale |
| Qalabka Baaritaanka (SynKit) | ES-D-AADH-1700 | set of 17 D-amino Acid Dehydrogenases, 1 mg 17 shay kasta * 1mg / shay |
★ gaar ahaan substrate-ka sare.
★ Xulasho xoog leh oo chiral ah.
★ beddelaad sare.
★ Alaabooyin ka yar.
★ Xaalado falcelin fudud.
★ deegaan ahaan.
➢ Caadiyan, habka falcelinta waa in uu ku jiraa substrate, xal buffer, enzyme, coenzyme iyo coenzyme nidaamka dib u soo kicinta (tusaale gulukoosta iyo gulukoosta dehydrogenase).
➢ D-AADH waa in lagu daraa ugu danbeyn habka falcelinta, ka dib marka pH iyo heerkulka lagu hagaajiyo xaalada falcelinta.
Tusaalaha 1 (Yaree α Ketoacids oo u dhigma D-amino acids)(1):
Ka dhig 2 sano ka hooseeya -20 ℃.
Weligaa ha la xiriirin xaaladaha ba'an sida: heerkulka sare, pH sare/hoose iyo dareeraha organic-fiirsashada sare leh.
1. Akita H, Hayashi J, Sakuraba H, iyo al.Xuduudaha Microbiology, 2018, 9: 1760.
