Nucloside phosphoryalse (NP)
Enzymes | Koodhka badeecada | Tilmaamid |
Budada Enzyme | ES-NP-101~ ES-NP-103 | 3 Nuclosidephosphoryalse, 50 mg 3 walxood * 50mg / shayga, ama tiro kale |
Habka falcelinta soo socota ayaa loo isticmaali karaa tixraac:
1-10 mg/ml Substrate
10 mg/ml enzyme
100 mM Fosfate kaydiye (pH7.0)
Ku kicin falcelinta heerkulbeeg joogto ah (tusaale, 30oC, 150rpm) gudaha 12 ~ 24 saacadood.Centrifuge isku darka kasta si uu u soo dejiyo borotiinka, iyaga ka sarreeya waxaa loo isticmaali karaa falanqaynta TLC, HPLC ama GC.
Halkan ku qor fariintaada oo noo soo dir