Sawirka Muuqda ee Nukloside Phosphoryalse (NP)

Fosfooraska Nukloside (NP)

Sharaxaad Gaaban:

Ku saabsan fosfoorasyada nucleoside

Waxaa jira 3 nooc oo ah badeecooyinka NP ensaymka (Lambarka sida ES-NP-101~ ES-NP-103) oo ay samaysay SyncoZymes. ES-NP-101 waa purine nucleoside phosphorylase, ES-NP-102 iyo ES-NP-103 waa pyrimidine nucleoside phosphorylase. Nucleosidephosphorylase waxay u kala qaybin kartaa nucleosides saldhigyo iyo pentose phosphate. Nucleoside phosphorylase waxaa loo qaybin karaa purine nucleoside phosphorylase iyo pyrimidine nucleoside phosphorylase iyadoo loo eegayo doorbidka saldhigyada nucleoside. Purine nucleoside phosphorylase wuxuu metaabi karaa adenosine ilaa adenine, inosine ilaa hypoxanthine, guanosine ilaa guanine, wuxuuna soo saari karaa ribose phosphate isku mar. Pyrimidine nucleoside phosphorylase wuxuu metaabi karaa uridine una gudbin karaa uracil wuxuuna soo saari karaa ribose phosphate.

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Faahfaahinta Badeecada

Calaamadaha Alaabta

Nooca falgalka fosfoorasyada Nukloside:

Macluumaadka Badeecada:

Ensaymyada	Koodhka Badeecada	Faahfaahinta
Budada Ensaymka	ES-NP-101~ ES-NP-103	set ah 3 Nuclosidephosphoryalse, 50 mg midkiiba 3 shay * 50mg / shay, ama tiro kale

Habka falgalka baaritaanka fosfooraska Nukloside:

Nidaamka falcelinta soo socda ayaa loo isticmaali karaa tixraaca:
1-10 mg/ml Substrate
10 mg/ml Ensaym
100 mM Fosfate baffer (pH7.0)
Ku shid falgallada mashiinka heerkulka joogtada ah (tusaale ahaan, 30oC, 150rpm) muddo 12-24 saacadood ah. Ku shub isku dar kasta si uu borotiinka u soo daadiyo, kuwaas oo ah kuwa ugu sarreeya waxaa loo isticmaali karaa falanqaynta TLC, HPLC ama GC.