Oxynitrilases (HNL)

Sharaxaad Gaaban:

Ku saabsan oxynitrilases

ES-HNLs: Ensaym fasal ah oo si xushmad leh u kiciya ku darista HCN aldehydes (ketones) si loo helo alkoholka cyanide nooca R ama S, kuwaas oo si fudud loogu beddeli karo noocyo badan oo daroogo ama daawooyin ah iyada oo loo marayo habka kiimikada.
Waxaa jira 29 nooc oo ah badeecooyinka oxynitrilase (Lambarka ES-HNL-101～ES-HNL-129) oo ay samaysay SyncoZymes. SZ-HNL waa qalab waxtar leh oo loogu talagalay isku-darka (R)-cyanohydrins ama (S)-cyanohydrins oo ka yimaada noocyo kala duwan oo aldehydes udgoon, aliphatic iyo heterocyclic ah ama xitaa ketones.
Nooca falgalka kicinta:

Oxynitrilases HNL2

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Faahfaahinta Badeecada

Calaamadaha Alaabta

Macluumaadka Badeecada:

Ensaymyada	Koodhka Badeecada	Faahfaahinta
Budada Ensaymka	ES-HNL-101~ ES-HNL-129	set ah 29 oxynitrilases, 50 mg midkiiba 29 shay * 50mg / shay, ama tiro kale
Qalabka Baaritaanka (SynKit)	ES-HNL-1800	set ah 18 (S)-oxynitrilases, 1 mg midkiiba 18 shay * 1mg / shay
Qalabka Baaritaanka (SynKit)	ES-HNL-1100	set ah 11 (R)-oxynitrilases, 1 mg midkiiba 11 shay * 1mg / shay

Faa'iidooyinka:

★ Gaar ahaansho sare oo substrate ah.
★ Xulasho xooggan oo ku saabsan chiral.
★ Beddel sare.
★ Wax soo saar yar.
★ Xaalado falcelin fudud.
★ Deegaanka u wanaagsan.

Tilmaamaha isticmaalka:

➢ Caadiyan, nidaamka falgalka waa inuu ku jiraa substrate (aldehydes / ketones, HCN), xalka kaydka (pH falcelinta ugu fiican) iyo enzymes-ka.
➢ Dhammaan ES-HNL-yada waxaa lagu tijaabin karaa si kala duwan nidaamka falcelinta ee kor ku xusan ama Qalabka Baaritaanka HNL (SynKit HNL).
➢ Dhammaan noocyada ES-HNL ee u dhigma xaaladaha kala duwan ee falcelinta ugu wanaagsan waa in si gaar ah loo barto.
➢ Substrate-ka ama badeecada leh feejignaan sare ayaa laga yaabaa inay joojiso dhaqdhaqaaqa ES-HNL. Si kastaba ha ahaatee, joojinta waxaa lagu yareyn karaa iyadoo lagu daro substrate-ka dufcaddii.