Hydroaminase (HAM)
Sharaxaad Gaaban:
Ku saabsan Hydroaminase
Waxaa jira 2 nooc oo alaabada enzyme HAM ah (Lambarka sida ES-HAM-101 ~ ES-HAM-102) oo ay soo saartay SyncoZymes.HAM waxay kicin kartaa ammonium acid enoic ama asalkeeda si ay u soo saarto amino acids chiral.HAM waxaa loo isticmaali karaa in lagu soo saaro amino acids chiral (ama amines chiral) oo laga helo asiidhyada enoic (ama alkenes).Ku-deeqaha Ammonia, sida biyaha ammonia ama cusbada ammonium, waa lagama maarmaan.
Nooca falcelinta katalytik ee Hydroaminase
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iimayl:lchen@syncozymes.com
| Enzymes | Koodhka badeecada | Tilmaamid |
| Budada Enzyme | ES-HAM-101~ ES-HAM-102 | 2 Nuclosidephosphoryalse, 50 mg 2 walxood * 50mg / shayga, ama tiro kale |
Habka falcelinta soo socota ayaa loo isticmaali karaa tixraac:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): qaboojiyaha dabiiciga ah (1)
10% (v/v): Ammonia
Ku hagaaji pH ilaa 9.0 ka hor intaadan ku darin enzyme, iyo insaymiska waa in lagu daraa ugu dambeyntii.Ku kicin falcelinta heerkulbeeg joogto ah (tusaale, 30oC, 150rpm) gudaha 24 ~ 48 saacadood.Centrifuge isku darka kasta si uu u soo dejiyo borotiinka, iyaga ka sarreeya waxaa loo isticmaali karaa falanqaynta TLC, HPLC ama GC.
Fiiro gaar ah (1): Walxaha dabiiciga ah (sida DMSO, methanol, acetonitrile, IPA, iwm.) waa ikhtiyaari, oo loo isticmaalo substrate-ka aan la milmin.
