Amidase (AMD)
Enzymes:Waa kicinta bayooloji ee macromolecular, inta badan enzymes waa borotiinno
Amidase:Catalyze hydrolysis of endogenous kala duwan iyo aliphatic aliphatic iyo amides udgoon kala duwan by wareejinta koox acyl biyaha la soo saarka ee acids lacag la'aan ah iyo ammonia.Hydroxamic acids iyo asiidhyada kale ee dabiiciga ah ayaa si weyn loogu isticmaalaa dawo ahaan sababtoo ah waxay ka kooban yihiin arrimaha koritaanka, antibiyootiga iyo xakameynta burooyinka.Amidases-yada waxaa loo qaybin karaa nooca R iyo nooca S acylases iyadoo loo eegayo stereoselectivity kicinta.
Marka lagu daro kicinta hydrolysis ee amides, amidase waxay sidoo kale kicin kartaa falcelinta wareejinta acyl ee joogitaanka co-substrates sida hydroxylamine.
Amidase oo leh ilo kala duwan ayaa leh sifooyin kala duwan oo substrate ah, qaar ka mid ah ayaa kaliya hydrolyze kara amides caraf udgoon, qaar iyaga ka mid ah ayaa kaliya hydrolyze kara aliphatic amides, iyo qaar hydrolyze α-ama ω-amino amides.Inta badan amines-yadu waxay leeyihiin firfircooni firfircoon oo wanaagsan oo kaliya oo loogu talagalay acyclic ama amides caraf udgoon fudud, laakiin aromatics kakan, heterocyclic amides, gaar ahaan amides leh beddelka ortho, guud ahaan waxay ku yar yihiin dhaqdhaqaaqa (kaliya dhowr enzymes ayaa muujinaya saameyn wanaagsan).
Farsamaynta catalytic:
| Enzymes | Koodhka badeecada | Koodhka badeecada |
| Budada Enzyme | ES-AMD-101~ ES-AMD-119 | qayb ka mid ah 19 amidases, 50 mg midkiiba 19 shay * 50mg / shayga, ama tiro kale |
| Qalabka Baaritaanka (SynKit) | ES-AMD-1900 | set of 19 amidases, 1 mg midkiiba 19 shay * 1mg / shayga |
★ gaar ahaan substrate-ka sare.
★ Xulasho xoog leh oo chiral ah.
★ Waxtarka beddelka sare.
★ Alaabooyin ka yar
★ Xaalado falcelin fudud.
★ deegaan ahaan.
➢ Baadhitaanka enzyme waa in lagu sameeyaa substrates gaar ah sababtoo ah gaar ahaan substrate-ka, oo la helo ensaym ka kaca substrate-ka bartilmaameedka leh ee saamaynta ugu fiican.
➢ Weligaa ha la xiriirin xaaladaha ba'an sida: heerkulka sare, pH sare/hoose iyo dareere organic leh oo leh feejignaan sare.
➢ Caadiyan, habka falcelinta waa in lagu daraa substrate, xal wax-ka-qabashada (PH-ga ugu fican ee enzyme).Co-substrates sida hydroxylamine waa inay joogaan nidaamka falcelinta wareejinta acyl.
➢ AMD waa in lagu daraa ugu danbeyn habka falcelinta oo leh pH falcelinta ugu wanaagsan iyo heerkulka.
➢ Dhammaan noocyada AMD waxay leeyihiin xaalado falcelin wanaagsan oo kala duwan, marka mid kasta oo iyaga ka mid ah waa in si gaar ah loo darso.
Tusaale 1(1):
Dhaqdhaqaaqa Hydrolysis ee Substrates Amide ee kala duwan
| Substrate | Dhaqdhaqaaq gaar ah μmols min-1mg-1 | Substrate | Dhaqdhaqaaq gaar ah μmols min-1mg-1 |
| Acetamide | 3.8 | ο-OH benzamide | 1.4 |
| Propionamide | 3.9 | p-OH benzamide | 1.2 |
| Lactamide | 12.8 | ο-NH2benzamide | 1.0 |
| Butyramide | 11.9 | p-NH2benzamide | 0.8 |
| Isobutyramide | 26.2 | ο-Toluamide | 0.3 |
| Pentanamide | 22.0 | p-Toluamide | 8.1 |
| Hexanamide | 6.4 | Nikotinamide | 1.7 |
| Cyclohexanamide | 19.5 | Isonicotinamide | 1.8 |
| Acrylamide | 10.2 | Picolinamide | 2.1 |
| Metacrylamide | 3.5 | 3-Phenylpropionamide | 7.6 |
| Prolinamide | 3.4 | Indol-3-acetamide | 1.9 |
| Benzamide | 6.8 |
Dareen-celinta waxaa lagu sameeyay 50mM sodium fosfate xalinta, pH 7.5, at 70 ℃.
| Amides | Hydroxylamine | Hydrazine |
| Acetamide | 8.4 | 1.4 |
| Propionamide | 18.4 | 3.0 |
| Isobutyramide | 25.0 | 22.7 |
| Benzamide | 9.2 | 6.1 |
Dareen-celinta waxaa lagu sameeyay 50mM sodium fosfate xalinta, pH 7.5, at 70 ℃.
Feejignaanta reagent la xidhiidha: amides, 100 mM (benzamide, 10 mM);hydroxylamine iyo hydrazine, 400 mM;enzyme 0.9 μM.
Tusaale 2(2):
Tusaale 3(3):
1. D'Abusco AS, Ammendola S., iyo al.Extremophiles, 2001, 5:183-192.
2. Guo FM, Wu JP, Yang LR, iyo al.Habka Biochemistry, 2015, 50 (8): 1400-1404.
3. Zheng RC, Jin JQ, Wu ZM, iyo al.Bioorganic Chemistry, 2017, Waxaa laga heli karaa onlayn 7.
