Esterase&Lipase (PLE&CALB)
Waxaa jira 26 nooc oo ah alaabada enzyme PLE (Lambarka ES-PLE-101～ES-PLE-126) oo ay soo saartay SyncoZymes.ES-PLE waxaa loo isticmaali karaa hydrolysis of aliphatic iyo xeryahooda ester, ama xallinta regioselective iyo stereoselective si loo farsameeyo asiidhyada chiral iyo kuwa ka soo jeeda.
Nooca falcelinta catalytic:
★ gaar ahaan substrate-ka sare.
★ Xulasho xoog leh oo chiral ah.
★ beddelaad sare.
★ Alaabooyin ka yar
★ Xaalado falcelin fudud.
★ deegaan ahaan.
➢ Caadiyan, habka falcelinta waa in lagu daraa substrate, xal-u-helid iyo ES-PLE.Esterification ee ES-PLE-yada qaarkood waxaa lagu fuliyaa marxaladda organic.
➢ Dhammaan noocyada ES-PLE ee u dhigma xaaladaha kala duwan ee falcelinta ugu wanaagsan waa in si gaar ah loo daraaseeyaa.
Tusaale 1 (Biosynthesis of Pregabalin Intermediate)(1):
Tusaale 2(2):
Tusaale 3(3):
Tusaale 4(4):
Ka dhig 2 sano ka hooseeya -20 ℃.
Waligaa ha la xiriirin xaaladaha daran sida: heerkulka sare, pH sare/hoose.
1. Xu FX, Chen SY, Xu G, iyo Tal.Appl.Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD iyo tal.J. AmChem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal.Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron: Asymmetry 2000, 11, 917
