Haydaroaminase (HAM)
Sharaxaad Gaaban:
Ku saabsan Haydaroaminase
Waxaa jira 2 nooc oo ah badeecada ensaymka HAM (Lambarka ES-HAM-101~ ES-HAM-102) oo ay samaysay SyncoZymes. HAM wuxuu kicin karaa ammonia-ka enoic acid ama waxyaabaha ka soo baxa si uu u soo saaro ammonia-ka chiral. HAM waxaa loo isticmaali karaa in lagu sameeyo ammonia-ka chiral (ama ammonia-ka chiral) asiidhyada enoic (ama alkenes). Deeq-bixiyaha ammonia, sida biyaha ammonia ama milixda ammonia, waa lagama maarmaan.
Nooca falgalka kicinta ee Hydroaminase
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Iimayl:lchen@syncozymes.com
| Ensaymyada | Koodhka Badeecada | Faahfaahinta |
| Budada Ensaymka | ES-HAM-101~ ES-HAM-102 | set ah 2 Nuclosidephosphoryalse, 50 mg midkiiba 2 shay * 50mg / shay, ama tiro kale |
Nidaamka falcelinta soo socda ayaa loo isticmaali karaa tixraaca:
1-10 mg/ml: Substrate
10 mg/ml: Ensaym
10% (v/v): Isku-darka dabiiciga ah (1)
10% (v/v): Ammooniya
Ku hagaaji pH-ga 9.0 ka hor inta aadan ku darin ensaym, ensaym-kana waa in lagu daraa ugu dambeyntii. Falcelinta ku shid qalab heerkulbeeg joogto ah (tusaale ahaan, 30oC, 150rpm) muddo 24 ~ 48 saacadood ah. Ku dhex geli isku darka kasta si uu borotiinka u soo saaro, kuwaas oo ah kuwa ugu sarreeya ee loo isticmaali karo falanqaynta TLC、HPLC ama GC.
Fiiro gaar ah (1): Dareeraha dabiiciga ah (sida DMSO, methanol, acetonitrile, IPA, iwm.) waa ikhtiyaari, waxaana loo isticmaalaa substrate-yada aan milmi karin.
