Leucine Dehydrogenase (LeuDH)
Sharaxaad Gaaban:
Ku saabsan Leucine dehydrogenase
Waxaa jira 1 nooc oo ah badeecada enzyme LeuDH (Lambarka ES-LeuDH-101) ee ay soo saartay SyncoZymes.LeuDH waxay kicin kartaa ammoniation-ka yar ee 4-methylbutanoic acid (milix) ilaa L-leucine ama oksaydhka leucine ilaa 4-methylbutanoic acid.ES-LeuDH waxay sidoo kale kicin kartaa hamiga yaraynta asiidhyada α-keto ee leh qaab dhismeed la mid ah si loo diyaariyo soosaarayaasha amino acid ee u dhigma.Inta lagu jiro nidaamka catalysis, NADH waxay lagama maarmaan u tahay gaadiidleyda hydrogen.
Nooca falcelin dehydrogenasekalytic Leucine
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iimayl:lchen@syncozymes.com
| Enzymes | Koodhka badeecada | Tilmaamid |
| Budada Enzyme | ES-LeuDH-101 | 1 Monoamine Oxidase, 50 mg 1 shay * 50mg / shay, ama tiro kale |
Habka falcelinta soo socota ayaa loo isticmaali karaa tixraac:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): qaboojiyaha dabiiciga ah (1)
100 mM: Fosfate kaydiye (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Ku kicin falcelinta heerkulka gariiraha joogtada ah (tusaale, 30oC, 150rpm) gudaha 24 ~ 48 saacadood.Centrifuge isku darka kasta si uu u soo dejiyo borotiinka, iyaga ka sarreeya waxaa loo isticmaali karaa falanqaynta TLC, HPLC ama GC.
Xusuusin (1): Walxaha dabiiciga ah (sida DMSO, methanol, acetonitrile, IPA, iwm.) waa ikhtiyaari, oo loo isticmaalo substrate-ka aan la milmin.
