Leucine Dehydrogenase (LeuDH)
Sharaxaad Gaaban:
Ku saabsan Leucine dehydrogenase
Waxaa jira 1 nooc oo ah wax soo saarka ensaymka LeuDH (Lambarka ES-LeuDH-101) oo ay samaysay SyncoZymes. LeuDH waxay kicin kartaa ammonia-ka yareeya ee 4-methylbutanoic acid (cusbo) ilaa L-leucine ama oksaydhka leucine ilaa 4-methylbutanoic acid. ES-LeuDH sidoo kale waxay kicin kartaa ammonia-ka yareeya ee asiidhyada α-keto oo leh qaab-dhismeed la mid ah si loo diyaariyo waxyaabaha ka soo baxa amino acid ee u dhigma. Inta lagu jiro habka catalysis-ka, NADH waa lagama maarmaan sida qaadaha haydarojiin.
Nooca falgalka katalytic-ga ee Leucine dehydrogenase
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Iimayl:lchen@syncozymes.com
| Ensaymyada | Koodhka Badeecada | Faahfaahinta |
| Budada Ensaymka | ES-LeuDH-101 | set 1 Monoamine Oxidase ah, 50 mg midkiiba 1 shay * 50mg / shay, ama tiro kale |
Nidaamka falcelinta soo socda ayaa loo isticmaali karaa tixraaca:
1-10 mg/ml: Substrate
10 mg/ml: Ensaym
10% (v/v): Isku-darka dabiiciga ah (1)
100 mM: Baafiyaha Fosfate (pH7.0)
0.2 mg/ml: NAD+
100 mM: Qaab-dhismeedka Ammonium
10 mg/ml: Qaabaynta dehydrogenase (FDH)
Ku shid falgallada mashiinka heerkulka joogtada ah (tusaale ahaan, 30oC, 150rpm) muddo 24 ~ 48 saacadood ah. Ku shub isku dar kasta si uu borotiinka u soo daadiyo, kuwaas oo ah kuwa ugu sarreeya waxaa loo isticmaali karaa falanqaynta TLC, HPLC ama GC.
Fiiro gaar ah (1): Dareeraha dabiiciga ah (sida DMSO, methanol, acetonitrile, IPA, iwm.) waa ikhtiyaari, waxaana loo isticmaalaa substrate-yada aan milmi karin.
