Ene reductase (ERED)
ES-EREDs waxay kiciyaan noocyada kala duwan ee substrate-ka sababtoo ah noocyada kala duwan ee substrate-ka. Guud ahaan, C=C ee α, β-unsaturated compounds oo leh kooxaha nuuga elektarooniga (oo ay ku jiraan ketone aldehyde, kooxaha nitro, asiidhyada carboxylic, esters, anhydride, lactones, imines, iwm.) si fudud ayaa loo dhimaa ES-EREDs, laakiin isku-xidhka labanlaabka ah ee aan firfircoonayn ma aha.
Waxaa jira 46 nooc oo ah badeecooyinka ensaymka ERED (Lambarka loo yaqaan ES-ERED-101~ES-ERED-146) oo ay samaysay SyncoZymes.
Habka kicinta:
| Ensaymyada | Koodhka Badeecada | Faahfaahinta |
| Budada Ensaymka | ES-ERED-101~ ES-ERED-146 | set ah 46 Ene Reductases, 50 mg midkiiba 46 shay * 50mg / shay, ama tiro kale |
| Qalabka Baaritaanka (SynKit) | ES-ERED-4600 | set ah 46 Ene Reductases, 50 mg midkiiba 46 shay * 50mg / shay, ama tiro kale |
★ Gaar ahaansho sare oo substrate ah.
★ Xulasho xooggan oo ku saabsan chiral.
★ Beddel sare.
★ Wax soo saar yar.
★ Xaalado falcelin fudud.
★ Deegaanka u wanaagsan.
★ Badbaado sare.
➢ Caadiyan, nidaamka falcelinta waa inuu ku jiraa substrate, xalka kaydka (pH falcelinta ugu fiican), coenzymes (NAD(H) ama NADP(H)), nidaamka dib u soo noolaynta coenzyme (tusaale ahaan gulukooska iyo glucose dehydrogenase) iyo ES-ERED.
➢ Dhammaan ES-ERED-yada waxaa lagu tijaabin karaa si kala duwan nidaamka falcelinta ee kor ku xusan ama Qalabka Baaritaanka ERED (SynKit ERED).
➢ Dhammaan noocyada ES-ERED ee u dhigma xaaladaha kala duwan ee falcelinta ugu wanaagsan waa in si gaar ah loo darso.
➢ Substrate-ka ama badeecadda leh feejignaan sare ayaa laga yaabaa inay joojiso dhaqdhaqaaqa ES-ERED. Si kastaba ha ahaatee, joojinta waxaa lagu yareyn karaa iyadoo lagu daro substrate-ka dufcaddii.
Tusaale 1(α,β-unsaturated aldehydes ama ketones)(1):
Tusaale 2(α,β-acids carboxylic ah iyo waxyaabaha ay ka soo saaraan)(2):
Ku hay heerkul ka hooseeya -20 ℃ 2 sano.
Weligaa ha taaban xaalado daran sida: heerkul sare, pH sare/hoose iyo dareere dabiici ah oo xoog badan.
1. Lucídio C, Fardelone J, Augusto R, iyo tal. J.Mol.Catal.B:Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, iyo tal. .Org.Lett, 2007, 9(26): 5409-5411.
