Nitrile hydratase (NHT)
Sharaxaad Gaaban:
Ku saabsan ES-NHTs
ES-NHTs: nooc ka mid ah metalloenzyme, kaas oo si gaar ah u milma nitriles una geliya amide-ka aasaasiga ah ee u dhigma. Si weyn ayaa loogu isticmaalaa ma aha oo kaliya isku-darka amino acids, amides, carboxylic acids iyo waxyaabaha ka soo baxa, laakiin sidoo kale diyaarinta daawooyinka chiral. Waxaa jira 24 nooc oo nitrile hydratases ah oo ay samaysay SyncoZymes (Lambarka sida ES-NHT-101～ES-NHT-124).
Nooca falgalka kicinta:
Moobayl/Wechat/WhatsApp: +86-13681683526
Iimayl:lchen@syncozymes.com
| Ensaymyada | Koodhka Badeecada | Faahfaahinta |
| Budada Ensaymka | ES-NHT-101~ ES-NHT-124 | set ah 24 Nitrile Hydratases, 50 mg midkiiba 24 shay * 50mg / shay, ama tiro kale |
| Qalabka Baaritaanka (SynKit) | ES-NHT-2400 | set ah 24 Nnitrile Hydratases, 1 mg midkiiba 24 shay * 1mg / shay |
★ Gaar ahaansho sare oo substrate ah.
★ Xulasho xooggan oo ku saabsan chiral.
★ Beddel sare.
★ Wax soo saar yar.
★ Xaalado falcelin fudud.
★ Deegaanka u wanaagsan.
➢ Caadiyan, nidaamka falgalka waa inuu ku jiraa substrate, xalka kaydka (pH falcelinta ugu fiican) iyo ES-NHT.
➢ Dhammaan ES-NHT-yada waxaa lagu tijaabin karaa si kala duwan nidaamka falcelinta ee kor ku xusan ama Qalabka Baaritaanka NHT (SynKit NHT).
➢ Dhammaan noocyada ES-NHT ee u dhigma xaaladaha kala duwan ee falcelinta ugu wanaagsan waa in si gaar ah loo barto.
➢ Substrate-ka ama badeecada leh feejignaan sare ayaa laga yaabaa inay joojiso dhaqdhaqaaqa ES-NHT. Si kastaba ha ahaatee, joojinta waxaa lagu yareyn karaa iyadoo lagu daro substrate-ka dufcad ahaan, iyadoo lala xiriirinayo amidases-ka ama soo saarista badeecada.
Tusaale 1 (isku xidhka amidase)(1):
Tusaale 2(2):
Ku hay heerkul ka hooseeya -20 ℃ 2 sano.
Weligaa ha taaban xaalado daran sida: heerkul sare, pH sare/hoose iyo dareere dabiici ah oo xoog badan.
1 Vojtech V, Ludmila M, Alicja BV, iyo Tal. J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, iyo tal. Tetrahedron lett, 2010, 51: 1639-1641.
