Nitrile reductase (NRED)
Sharaxaad Gaaban:
Ku saabsan Nitrile reductase
Waxaa jira 3 nooc oo ah wax soo saarka ensaymka NRED (Lambarka ES-NRED101~ ES-NRED103) oo ay samaysay SyncoZymes. NRED waxay kicin kartaa hoos u dhigista kooxda nitrile una beddelaysaa amine. Inta lagu jiro geeddi-socodka katalisnimada, NADPH waxaa loo baahan yahay sidii qaade haydarojiin ah.
Nooca falgalka nitrile reductasecatalytic
Moobayl/Wechat/WhatsApp: +86-13681683526
Iimayl:lchen@syncozymes.com
| Ensaymyada | Koodhka Badeecada | Faahfaahinta |
| Faahfaahinta | ES- NRED -101~ ES- NRED -103 | set ah 3 Nitrile Reductase ah, 50 mg midkiiba 3 shay * 50mg / shay, ama tiro kale |
Nidaamka falcelinta soo socda ayaa loo isticmaali karaa tixraaca:
1-10 mg/ml Substrate
10 mg/ml Ensaym
10% (v/v) Isku-darka dabiiciga ah (1)
100 mM Fosfate baffer (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Gulukoos
2 mg/ml Gulukoos dehydrogenase (GDH)
Ku shid falgallada mashiinka heerkulka joogtada ah (tusaale ahaan, 30oC, 150rpm) muddo 24 ~ 48 saacadood ah. Ku shub isku dar kasta si uu borotiinka u soo saaro, kuwaas oo ah kuwa ugu sarreeya waxaa loo isticmaali karaa falanqaynta TLC、HPLC ama GC.
Fiiro gaar ah (1): Dareeraha dabiiciga ah (sida DMSO, methanol, acetonitrile, IPA, iwm.) waa ikhtiyaari, waxaana loo isticmaalaa substrate-yada aan milmi karin.
