Nitrile reductase (NRED)
Sharaxaad Gaaban:
Ku saabsan Nitrile reductase
Waxaa jira 3 nooc oo ah badeecada enzyme NRED (Lambarka sida ES-NRED101 ~ ES-NRED103) oo ay soo saartay SyncoZymes.NRED waxay kicin kartaa dhimista kooxda nitrile si loo amiinto.Inta lagu jiro nidaamka catalysis, NADPH ayaa loo baahan yahay sida gaadiid-qaade hydrogen ah.
Nooca falcelinta nitrile reductasecatalytic
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iimayl:lchen@syncozymes.com
| Enzymes | Koodhka badeecada | Tilmaamid |
| Tilmaamid | ES- NRED -101~ ES- NRED -103 | qayb ka mid ah 3 Nitrile Reductase, 50 mg 3 walxood * 50mg / shayga, ama tiro kale |
Habka falcelinta soo socota ayaa loo isticmaali karaa tixraac:
1-10 mg/ml Substrate
10 mg/ml enzyme
10% (v/v) Nasinta dabiiciga ah (1)
100 mM Fosfate kaydiye (pH7.0)
0.2 mg/ml NADP+
10 mg/ml gulukoos
2 mg/ml Glucose dehydrogenase (GDH)
Ku kicin falcelinta heerkulbeeg joogto ah (tusaale, 30oC, 150rpm) gudaha 24 ~ 48 saacadood.Centrifuge isku darka kasta si uu u soo dejiyo borotiinka, iyaga ka sarreeya waxaa loo isticmaali karaa falanqaynta TLC, HPLC ama GC.
Fiiro gaar ah (1): Walxaha dabiiciga ah (sida DMSO, methanol, acetonitrile, IPA, iwm.) waa ikhtiyaari, oo loo isticmaalo substrate-ka aan la milmin.
