Phosphokinase (PKase)
Enzymes | Koodhka badeecada | Tilmaamid |
Qalabka Baaritaanka (SynKit) | ES-PKase-101~ES-PKase-121 | 21 Ketoreductases, 1 mg midkiiba 21 shay * 1mg / shayga |
★ gaar ahaan substrate-ka sare.
★ Xulasho xoog leh oo chiral ah.
★ beddelaad sare.
★ Alaabooyin ka yar
★ Xaalado falcelin fudud.
★ deegaan ahaan.
➢ Caadiyan, habka falcelinta waa in ay ku jiraan substrate, xal buffer, enzyme, ATP, Mg2+.
➢ PKase waa in lagu daraa ugu danbeyn habka falcelinta, ka dib marka pH iyo heerkulka lagu hagaajiyo xaalada falcelinta.
Tusaale 1 (Synthesis of nicotinamide ribose phosphate from nicotinamide ribose)(1):
Fiiro gaar ah: Tusaalooyinka codsiga iyo tixraacyada waxaa loogu talagalay in lagu muujiyo baaxadda codsiga ee PCAse si loo fududeeyo fahamka, oo aan u dhigmin ensaymka gaarka ah ee SyncoZymes.
Ka dhig 2 sano ka hooseeya -20 ℃.
Weligaa ha la xiriirin xaaladaha ba'an sida: heerkulka sare, pH sare/hoose iyo dareeraha organic-fiirsashada sare leh.
1. Khan, Javed A., Song Xiang, iyo Liang Tong.Qaab dhismeedka 15.8 (2007): 1005-1013.