Transaminase (ATA)
Ensaymyada: Waa kiciyayaal bayooloji ah oo macromolecular ah, inta badan enzymes-ku waa borotiinno.
Transaminases: Nooc ka mid ah ensaymada oo kiciya amino-ka u kala gudba amino acids-ka iyo keto acids-ka. Transaminases-ku waa ensaymo bayooloji oo muhiim ah oo ku jira isku-darka aan sinnayn iyo xallinta jinsiyadda ee amiinada chiral.
Aminotransferase waxaa loo qaybin karaa afar fasal iyadoo loo eegayo taxanaha iyo qaab-dhismeedka: Ⅰ, Ⅱ, Ⅲ iyo Ⅳ. ω-aminotransferases waxay ka tirsan yihiin transaminases-ka fasalka II, oo badanaa loo isticmaalo diyaarinta chiral amines iyo amino acids aan dabiici ahayn, sida β-amino acids.
ω-aminotransferases: Inta badan, ω-transaminase waxaa loola jeedaa fasal ensaymo ah, oo ah falgallada wareejinta ammonia ee catalytic iyada oo aan lahayn α-amino acid sida substrate ama badeecad.
Habka kicinta:
| Ensaymyada | Koodhka Badeecada | Koodhka Badeecada |
| Budada Ensaymka | ES-ATA-101~ ES-ATA-165 | set ah 65 ω-Transaminases, 50 mg midkiiba 65 shay * 50mg / shay, ama tiro kale |
| Qalabka Baaritaanka (SynKit) | ES-ATA-6500 | set ah 65 ω-Transaminases, 1 mg midkiiba 65 shay * 1mg / shay |
★ Gaar ahaansho sare oo substrate ah.
★ Xulasho xooggan oo ku saabsan chiral.
★ Waxtarka sare ee beddelka.
★ Wax soo saar yar.
★ Xaalado falcelin fudud.
★ Deegaanka u wanaagsan.
➢ Baaritaanka ensaymka waa in loo sameeyaa substrate-yo gaar ah sababtoo ah gaar ahaanshaha substrate-ka, oo la helo ensaym kiciya substrate-ka bartilmaameedka leh saameynta katalytic-ga ugu fiican.
➢ Weligaa ha taaban xaalado daran sida: heerkul sare, pH sare/hoose iyo dareere dabiici ah oo leh xoog badan.
➢ Caadiyan, nidaamka falcelinta waa inuu ku jiraa substrate, xalka kaydka, deeq-bixiyaha amino (sida amino acids iyo 1-phenyl ethylamine) ama receptor (sida keto acids), coenzyme (PLP), cosolvent (sida DMSO).
➢ ATA waa in lagu daraa habka falcelinta ugu dambeeya, ka dib marka pH iyo heerkulka la hagaajiyo xaaladda falcelinta.
➢ Dhammaan noocyada ATA waxay leeyihiin xaalado kala duwan oo falcelin ah oo ugu wanaagsan, sidaa darteed mid kasta oo ka mid ah waa in si gaar ah loo bartaa.
Tusaale 1(isku-darka Sitagliptin, isku-darka aan sinnayn)(1):
Tusaale 2 (Mexiletine, Isku-darka xallinta kinetic-ga oo leh isku-darka aan sinnayn)(2):
1 Savile CK, Janey JM, Mundorff EC, et al. Sayniska, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, iyo al. Xarfaha dabiiciga ah, 2009,11 (21): 4810-4812.
