Cytochrome P450 monooxygenase (CYP)

Sharaxaad Gaaban:

Ku saabsan CYP

ES-CYPs: Nooc ka mid ah ensaymada redox oo leh mercaptan-heme oo ah xarunta firfircoon si loo kiciyo wareejinta oksijiinta. Waa oksijiin-ka-baxa ee terminal-ka waxayna u baahan yihiin heme si ay u dhaqaajiso oksijiinta.

Cytochrome P450 monooxygenase wuxuu kiciyaa oksaydhka, epoksaydhka iyo hydroxylation ee alkyl, hydroxylation iyo oksaydhka ammonia, oksaydhka sulfur, dealkylation (oxygen, sulfur, ammonia), oksaydhka dehydrogenation, deamination iyo dehalogenation.
Waxaa jira 8 nooc oo ah badeecooyinka cytochrome P450 monooxygenase (Lambarka ES-CYP-101～ES-CYP-108) oo ay samaysay SyncoZymes.

Moobayl/Wechat/WhatsApp: +86-13681683526

Iimayl:lchen@syncozymes.com

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Faahfaahinta Badeecada

Calaamadaha Alaabta

Ku saabsan CYP:

Nooca falgalka kicinta:

Macluumaadka Badeecada:

Ensaymyada	Qalabka Baaritaanka (SynKit)	Faahfaahinta
Budada Ensaymka	ES-CYP-101~ ES-CYP-108	set ah 8 Cytochrome P450 Monooxygenases, 50 mg midkiiba 8 shay * 50mg / shay, ama tiro kale
Qalabka Baaritaanka (SynKit)	ES-CYP-800	set ah 8 Cytochrome P450 Monooxygenases, 1mg midkiiba 8 shay * 1mg / shay

Faa'iidooyinka:

★ Baaxadda ballaaran ee substrate-ka.
★ Beddel sare.
★ Wax soo saar yar.
★ Xaalado falcelin fudud.
★ Deegaanka u wanaagsan.

Tilmaamaha isticmaalka:

➢ Caadiyan, nidaamka falcelinta waa inuu ku jiraa substrate, xalka kaydka (PH-ga falcelinta ugu fiican ee ensaymka), coenzyme (NAD(H) ama NADP(H)), nidaamka dib-u-cusboonaysiinta coenzyme (tusaale ahaan gulukooska iyo gulukooska dehydrogenase) iyo nidaamka dib-u-cusboonaysiinta coenzyme iyo coenzyme-ka ES-CYP waxaa lagu beddeli karaa hydrogen peroxide nidaamka falgalka qayb ahaan.
➢ Dhammaan noocyada ES-CYP ee u dhigma xaaladaha kala duwan ee falcelinta ugu wanaagsan waa in si gaar ah loo darso.
➢ Substrate-ka ama badeecada leh feejignaan sare ayaa laga yaabaa inay joojiso dhaqdhaqaaqa ES-CYP. Si kastaba ha ahaatee, joojinta waxaa lagu yareyn karaa iyadoo lagu daro substrate-ka dufcaddii.

Tusaalooyinka Codsiga:

Tusaale 1aad⁽¹⁾:

Tusaale 2⁽²⁾:

Tusaale 3⁽³⁾:

Tusaale 4⁽⁴⁾:

Kaydinta:

Ku hay heerkul ka hooseeya -20 ℃ 2 sano.

Feejignaan:

Weligaa ha taaban xaalado daran sida: heerkul sare, pH sare/hoose iyo dareere dabiici ah oo xoog badan.

Tixraacyada:

1. Zaretzki J, Matlock M, iyo Swamidass S JJ Chem. Inf. Model, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez F A., iyo Tal. J. Am Chem. Soc., 2006, 128 (26), 8374-8375.
3. Cryle M J., Matovic N J., iyo De Voss J J. Org. Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal. Biochim. Biophys Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.; Cheon, WK iyo tall. Acta Crystallogr. 2009, 65, 372.