Epoxide hydrolase (EH)
Sharaxaad Gaaban:
Ku saabsan epoxide hydrolase
EH waa ether hydrolase kaas oo kicinaya hydrolysis stereoselective ee epoxides jinsiyadeed si ay u soo saaraan 1, 2-diol iyo epoxides muuqaal ahaan firfircoon, kuwaas oo loo isticmaali karo xallinta chiral ee epoxides ama diyaarinta diols chiral.Waxaa si weyn loogu isticmaalaa daawada iyo qaybaha kale.Waxaa jira 3 nooc oo ah alaabada epoxide hydrolases (Lambarka sida ES-EH-101～ES-EH-103) oo ay soo saartay SyncoZymes.ES-EH waa qalab faa'iido leh oo lagu kicinayo isku-darka alkolada chiral iyada oo loo marayo hydrolysis stereoselective ee aliphatic iyo aromatic epoxides.
Nooca falcelinta catalytic:
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iimayl:lchen@syncozymes.com
| Enzymes | Koodhka badeecada | Tilmaamid |
| Budada Enzyme | ES-EH-101~ ES-EH-103 | 3 Epoxide Hydrolases, 50 mg 3 walxood * 50mg / shayga, ama tiro kale |
| Qalabka Baaritaanka (SynKit) | ES-EH-300 | 3 Epoxide Hydrolases ah, 1 mg 3 walxood * 1mg / shayga |
★ gaar ahaan substrate-ka sare.
★ Xulasho xoog leh oo chiral ah.
★ beddelaad sare.
★ Alaabooyin ka yar
★ Xaalado falcelin fudud.
★ deegaan ahaan.
➢ Caadiyan, habka falcelinta waa in lagu daraa substrate, xallinta wax-ka-qabashada (pH) iyo ES-EH.
➢ Dhammaan noocyada ES-EH ee u dhigma xaaladaha kala duwan ee falcelinta ugu wanaagsan waa in si gaar ah loo darso.
➢ Substrate-ka sare u kaca ama badeecada leh ayaa laga yaabaa inay joojiyaan dhaqdhaqaaqa ES-EH.Si kastaba ha ahaatee, xannibaadda waxaa lagu nafisi karaa marka lagu daro qayb ka mid ah substrate.
Tusaale 1(1):
Ka dhig 2 sano ka hooseeya -20 ℃.
Weligaa ha la xiriirin xaaladaha ba'an sida: heerkulka sare, pH sare/hoose iyo dareeraha organic-fiirsashada sare leh.
1. Kim YH, Lee I, Choi SH, iyo Tal.J Mol Catal B-Enzym, 2013, 89: 48-51.
